Enzo Life Sciences的ROS-ID® Total ROS/Superoxide detection kit包括兩種熒光染料作為主要成分：非熒光、可滲透細胞的氧化應激檢測試劑（綠色，Ex/Em 490/525 nm）與多種活性物質（過氧化氫、過氧亞硝酸根和羥基）直接反應，產生綠色熒光產物。超氧化物檢測試劑（橙色，Ex/Em 550/620 nm）是一種可滲透細胞的探針，可與超氧化物特異性反應，產生橙色熒光產物。試劑盒還提供Pycyanin（ROS誘導劑）和NAC（ROS抑制劑）。
產品特點
● 用于檢測活細胞中超氧化物的產生
● 與細胞培養(yǎng)基的主要成分（酚紅，FBS和BSA）兼容
● 已通過顯微鏡、流式細胞儀和微孔板等應用驗證
實驗示例
圖1. 在加入了ROS/超氧化物檢測試劑并用綠膿菌素處理的 HeLa 細胞中，通過熒光顯微鏡對活性氧的形成進行了分析。在綠色通道中監(jiān)測一般氧化應激水平，而在橙色通道中檢測超氧化物的產生。用 NAC（一種通用的 ROS 抑制劑）預處理可防止 ROS 的形成。
圖2. 使用ROS-ID® Total ROS/Superoxide detection kit和ROS清除劑/抑制劑分析用抗-霉-素A（AMA，特異性超氧化物誘導劑）、叔丁基-過氧化物（TBHP，特異性過氧化物誘導劑）和綠膿菌素（通用ROS誘導劑）處理的HeLa細胞中ROS的產生。使用U-2 OS和CHO K1細胞獲得了類似的結果。
圖3. 通過流式細胞術分析Hela細胞中ROS的形成。數據以綠膿菌素（ROS/SO誘導劑）、TBHP（ROS 誘導劑）和 AMA（超氧化物誘導劑）處理后的陽性百分比表示。綠色柱表示ROS 陽性率%，橙色柱表示超氧化物陽性率%。
產品信息
產品貨號 | ENZ-51010 |
產品名稱 | ROS-ID® Total ROS/Superoxide detection kit/欣博盛生物 |
別名 | Reactive oxygen species / Superoxide |
規(guī)格 | 1*1Kit (200 fluorescence microscopy assays or 50 flow cytometry assays or 2x96 microplate assays) |
使用/穩(wěn)定性 | With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage. |
短期保存 | -20°C |
長期保存 | -80°C |
試劑盒組分 | Oxidative Stress Detection Reagent (Green), 300 nmoles Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 µmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack |
應用 | Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS |
部分產品引用文獻
1. Conditional Knockout of Hypoxia-Inducible Factor 1-Alpha in Tumor-Infiltrating Neutrophils Protects against Pancreatic Ductal Adenocarcinoma: J.L. Sieow, et al.; Int. J. Mol. Sci. 24, 753 (2023)
2. Antioxidant effect of nicotinamide mononucleotide in tendinopathy: K. Yamaura, et al.; BMC Musculoskelet. Disord. 23, 249 (2022)
3. Eicosatetraynoic Acid and Butyrate Regulate Human Intestinal Organoid Mitochondrial and Extracellular Matrix Pathways Implicated in Crohn’s Disease Strictures: I. Jurickova, et al.; Inflamm. Bowel Dis. 28, 988 (2022)
4. Hirsutella sinensis mycelium attenuates bleomycin-induced pulmonary inflammation and fibrosis in vivo: T.T. Huang, et al.; Sci. Rep. 5, 15282 (2022)
5. Inhibition of mitoNEET attenuates LPS-induced inflammation and oxidative stress: S. Lee, et al.; Cell Death Dis. 13, 127 (2022)
6. New glycoconjugation strategies for Ruthenium(II) arene complexes via phosphane ligands and assessment of their antiproliferative activity: D. Iacopini, et al.; Bioorg. Chem. 126, 105901 (2022)
7. Quercetin treatment protects the Achilles tendons of rats from oxidative stress induced by hyperglycemia: T. Yoshikawa, et al.; BMC Musculoskelet. Disord. 23, 563 (2022)
8. Areca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy: P.L. Wei, et al.; Int. J. Med. Sci. 18, 3452 (2021)
9. Differential oxidative stress responses and tobacco-specific nitrosamine accumulation in two burley varieties: J. Kurepa, et al.; J. Plant Physiol. 261, 153429 (2021)
10. Dihydroisotanshinone I induced ferroptosis and apoptosis of lung cancer cells: C.Y. Wu, et al.; Biomed. Pharmacother. 139, 111585 (2021)
11. Incense smoke-induced oxidative stress disrupts tight junctions and bronchial epithelial barrier integrity and induces airway hyperresponsiveness in mouse lungs: N. Yamamoto, et al.; Sci. Rep. 11, 7222 (2021)
12. Nano-encapsulation of hydroxytyrosol into formulated nanogels improves therapeutic effects against hepatic steatosis: An in vitro study: E. Mauri, et al.; Mater. Sci. Eng. C Mater. Biol. Appl. 124, 112080 (2021)
13. Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma: D. Witzigmann, et al.; J. Control Release 334, 138 (2021)
14. Polymer-ritonavir derivate nanomedicine with pH-sensitive activation possesses potent anti-tumor activity in vivo via inhibition of proteasome and STAT3 signaling: L. Sivák, et al.; J. Control Release 332, 563 (2021)
15. Selective striatal cell loss is ameliorated by regulated autophagy of the cortex: K. Cho & G.W. Kim; Life Sci. 282, 119822 (2021)
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